Detection of oncogenic human papilloma virus type 16 and 18 using loop-mediated isothermal amplification combined with lateral flow dipstick

High-risk human papillomavirus (HPV) infection is a major risk factor for cervical carcinogenesis, especially type 16 and 18. This cancer affect to deaths in the most woman. Although there is a vaccine to prevent but identify types of HPV in laboratory remain very important for vaccination. In the present, polymerase chain reaction (PCR) technique is gold standard for identifying HPV types but this technique is time-consuming and an expensive instrument for some laboratory. The aim of this study was to develop the platform for HPV-16 and HPV-18 detection using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD). The results showed that detection by LAMP-LFD techniques was comparable to agarose gel electrophoresis but using a shorter time with greater accuracy compared to turbidity. The results showed that LAMP-LFD techniques for detecting HPV type 16 and 18 gave 100% sensitivity at 100 viral copy number and no cross-reaction between other HPV types was observed. The results in clinical samples and interpretation by agarose gel showed the sensitivity were 91% and 82% for HPV type 16 and 18, respectively. The specificity was 100% in both HPV type 16 and 18. In addition, sensitivity and specificity were 87% and 100% respectively in HPV type 16 detection. The data indicated that efficacy of LAMP technique combined with LFD for detection of HPV is promising for further point-of-care diagnostic development.