Isolation identification and characterization of probiotic bacteria from infant’s feces for potential use as vaccine delivery vehicle

This study aimed to isolate, identify and characterize probiotic bacteria from breastfed-infant’s feces who was born by vagina delivery. These properties include hemolysis, tolerate to acid and bile salt, antimicrobial activity, antibiotic susceptibility, adhesion and cytotoxicity to intestinal cell. It was found that were four bacterial isolates include LW01, LW02, LW09 and LW10, showed good probiotic properties. Bacterial species identification by 16S rRNA sequence analysis of demonstrated that four isolates LW01, LW02, LW09 and LW10 were identified as Lactobacillus rhamnosus LW01, Lactobacillus rhamnosus LW02, Lactobacillus rhamnosus LW09 and Lactobacillus rhamnosus LW01. Electrotransformation of plasmid pLC13:9:pLDH-PRO1:GFPuv to L.rhamnosus LW10 isolate found that this plasmid was able to replicate and control the expression of express green fluorescent protein (GFP) gene in all L.rhamnosus isolates. For these candidate probiotic bacteria should be considered for the further advance study of probiotic properties in animal model and determination of genetic engineering for development of these bacterial isolates as vaccine delivery vector.